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KMID : 0350519960490020595
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Journal of Catholic Medical College
1996 Volume.49 No. 2 p.595 ~ p.610
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Expression of p53, Proliferating Cell Nuclear Antigen (PCNA), c-myc, and Epidermal Growth Factor Receptor (EGFR) in Cervical Neoplasia
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Abstract
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Expression of four biologic markers as p53, proliferating cell nuclear antigen (PCNA), c-myc, and epidermal growth factor receptor (EGFR), was studied immunohistochemically in 121 cases of cervical tissues, which included 15 cases of normal
cervical
tissues, 50 cases of squamous intraepithelial lesions (SIL), and 56 cases of invasive carcinomas. This study was designed to elucidate the roles of these factors in the genesis and progression of cervical neoplasia and to identify their
association
with
clinical parameters such as cell type, tumor size, lymph node involvement, squamous cell carcinoma (SCC) antigen level, and prognosis, In additon this study evaluated the differences of coexpression rates I normal cervical tissues, SILs, and
invasive
carcinomas.
1. The intensities of p53, c-myc, and EGFR expression and the rates of PCNA, c-myc, and EGFR expression in cervical SIL and invasive carcinomas were significantly higher than those in normal tissues (P<0.05) and P<0.01). But there were no
significant
differences between SILs and invasive carcinomas in both of intensities and rates of P53, PCNA, c-myc, and EGFR expression.
2. The intensities and rates of p53, PCNA, and c-myc expression in the invasive carcinomas did not correlate with clinical parameters including cell type, tumor size, lymph node involvement SCC antigen, and prognosis. But only the intensity and
rate of
EGFR expression were significantly higher in cases with tumor size larger than 3cm when it was compared with the cases with tumor sizes smaller than 3cm (P<0.010, in cases with lymph node involvement (P<0.05), and in cases with SCC antigen levels
more
than 2.5ng/ml when it was compared with the cases with SCC antigen levels less than 2.5ng/ml (P<0.05).
3. c-myc immunoreactivity was significantly correlated with EGFR overexpression (P<0.01).
4. The rates of simultaneous expression of four factors were decreased significantly in normal tissues and significantly increased in invasive carcinomas (P<0.01). The coexpression rate of two factors or more was 6.7% in SILs, and 70.0% in
invasive
carcinomas and the simultaneous expression ra5e of three factors or more was none in normal tissues, 34.0% in SILs, and 47.5% in invasive carcinomas.
There results show that the immunohistochemical detection of p53, PCNA, c-myc, and EGFR expression will be useful in differentiating the normal tissues and cervical neoplasia. The results of simultaneous immunohistochymical detection of these
four
factor expression also suggest to contribute a better understanding of the genesis of cervical carcinoma and that coexpression of more factors indicate more aggressiveness in cervical neoplasia, and show that simultaneous detection of these
factors
can
be used in the early detection of cervical neoplasia and to predict malignant transformation of the cervical lesions.
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KEYWORD
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